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Nonspecific binding was determined by incubating tissue sections in the incubation solution containing additionally 100 nM unlabeled GLP1(736)amide (Bachem, Bubendorf, Switzerland) that, at this concentration, completely and specifically displaces 125 I- GLP1(736)amide at the receptors

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However, it requires consistent sessions and proper aftercare

Use a 1 mL insulin syringe (2931 gauge, inch needle) for subcutaneous administration [9]

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