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DISCLAIMER: For in-vitro research only

Always wear sterile gloves, work on a clean, disinfected surface, and use sterile equipment

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IM is typically reserved for studies where the research objective is to observe the direct effect on a specific, isolated muscle or deep tissue area

There has never been a systematic study on the benefits and risks, he told Healthline

Some advanced users do split the dose (morning and evening) if taking higher amounts, but for most, a single daily dose is sufficient due to the relatively long half-life of NAD+ in cells