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After electrophoresis, the gels without loading proteins were cut, then blots including goal proteins were obtained according to protein markers, to be transferred to the membranes

doi: 10.1016/s0020-1693(00)82544-x

In Type 2 diabetes, this problem gets worse

In the body, about 95% of the total choline pool is converted to phosphatidylcholine, an essential component of the phospholipid bilayer and the predominant phospholipid in most mammalian cells