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The NHS weight loss plan focuses on sustainable lifestyle modifications, including: Calorie reduction through portion control and healthier food choices Increased physical activity (minimum 150 minutes moderate-intensity exercise weekly, plus muscle-strengthening activities at least twice weekly) [16] [17] Behavioural strategies and goal-setting Referral to specialist weight management services based on local criteria Referral thresholds for weight management services vary by locality

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Mice were housed in metabolic cages for 4 days before sacrifice

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114 In contrast to the stimulation of ATP production by low H 2 S levels, higher H 2 S concentrations inhibit complex IV of the mETC and thereby impair ATP production