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snRNA-seq and analysis For isolation of nuclei from gWAT, fresh adipose tissue was minced in nucleus preparation buffer (10 mM HEPES, 1.5 mM MgCl 2 , 10 mM KCl, 250 mM sucrose, 0.001% NP-40) with 0.2 U l Roche Protector RNase inhibitor followed by dounce homogenization

Practical considerations must be taken into account when preparing and handling peptides in solution

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Knockdown of GCH1 significantly activated ferritin autophagy upon erastin treatment, which was reversed by using an autophagy inhibitor

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One of the most important benefits of Carnipure L-Carnitine is its role in fat metabolism