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In this assay, GPx reduced cumene hydroperoxide while oxidizing GSH to GSSG

The biological significance of singlet oxygen is unclear

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When increasing concentrations of KA were added in the presence of L-dopa as the substrate, a similar mode of mixed inhibition was observed, with K I of 3.5 M and K IS of 150 M, similar to previous studies that reported K I values of 3.4, 5 and 4.7 M for mushroom tyrosinase 18,19,33