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Animal LAD and MCAO experiments For LAD and MCAO experiments, male C57BL/6 J mice aged 812 weeks were ordered from Charles River Laboratories UK (Margate, UK) and were housed in individually ventilated cages and maintained under controlled temperature (22 2 C) on a 12 h light/dark cycle with ad libitum access to chow and water

7.2 Global regulatory harmonization and social considerations An important challenge in the field of nanoparticle-based vaccines is achieving constant global regulatory standards

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