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These reagents were added either in Cys 2 -free culture medium or coincubated with BSO or erastin at indicated concentrations

Due to its defined mechanism and reproducible activity, 5-Amino-1MQ is commonly referenced in laboratory models exploring metabolic regulation, methyl donor balance, and NAD-related pathways

mitochondrial, SHMT2) catalyzes the transfer of the beta carbon of serine to tetrahydrofolate (THF) to form glycine and one-carbon units, 5,10-methylene-THF (Figure 1), which is critical for nucleotide synthesis

The amount of diluent, the type of diluent, and even the technique you use to introduce it can dramatically impact the outcome

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