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injections of 100 nmol kg 1 GLP-1MK-801, 10 nmol kg 1 semaglutide or vehicle (isotonic saline) based on baseline running wheel distance measurements, such that each group had similar average running wheel distances at the day of study initiation

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HPLC testing is an analytical method used to assess compound purity and verify product consistency through laboratory analysis

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Verified by independent HPLC and mass spectrometry, compared to the 98% industry threshold