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It was first characterized by Predrag Sikiric and colleagues at the University of Zagreb in the early 1990s, and a 2025 literature and patent review cataloged its effects across gastrointestinal, musculoskeletal, and neural models

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This can be achieved through various methods such as sonication, freeze-thaw cycles, enzymatic digestion (e.g., lysozyme treatment), or mechanical disruption (e.g., French press)

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