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Nevertheless, the observed associations do not establish a causal relationship between specific taxa and glycemic response

For protein purification, harvested cells were resuspended with ice-cold lysis buffer (50 mM Tris-Cl pH 8.0, 300 mM NaCl, and cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche)) at a ratio of 3.2 mL/g of cell pellet

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As the K4 and SPH domains belong to the and subunits of HGF, respectively, such strong K4/SPH interaction would be important for the structural integrity of HGF / heterodimer in addition to the disulfide bond between these two subunits