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Perfusate was collected from individual chambers at 2 min intervals and maintained at 4 C, and insulin concentrations were determined using insulin ELISA (MSD)

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Biochem Biophys Res Commun 2001;287:6670

To model natural-like GH pulses and IGF-1 activation in vitro and in vivo GH-axis studies

In Boyden chamber assays, TB4 stimulated keratinocyte migration 23-fold over medium alone within 45 hours with as little as 10 pg sufficient to produce a potent stimulatory response