Briefly, the storage solution was removed (5000 g , 2 min), and columns were washed with CH3CN (2 300 L, 5,000 g , 2 min), then equilibrated with buffer A (2 300 L, 5000 g , 2 min)
Intern Info Thibault Roudaire Functional Analysis of a Putative UDP-Glycosyltransferase Involved in the Biosynthesis of Cardiac Glucosides in Erysimum cheiranthoides Project summary: Cardiac glycosides are a class of defense-related plant metabolites that inhibit animal Na+, K+-ATPase membrane ion transporters, and thereby deter certain insect species from feeding or ovipositing on the plants that produce them
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