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Because muscles have a rich blood supply, this approach allows for rapid absorption, providing faster therapeutic effects than other delivery methods

For color development, 50 l/well TMB mixture was added and incubated for 5-10 minutes

Most modified peptides could be detected using both untargeted (DDA) and targeted (MRM) approaches, however the latter yielded better detection sensitivity with higher signal-to-noise and two sites were uniquely found by MRM

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