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However, in recent years there has been a boom in interest in using peptides for therapeutic purposes, from weight loss to anti-ageing and recovery from injury

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Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice

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