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Preventing adsorption: Mobile phases must include high ionic strength and neutral pH buffers to prevent secondary interactions with the packing material

While these dont occur in every case, they are a part of any complete discussion, and this is particularly true for the compound known as trimethylamine-N-oxide ( TMAO )

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Acta Derm Venereol 91:377 Lim HW et al

Administration of resistant maltodextrin improved gut barrier integrity and remarkably increased both the relative and combined abundance of commensals ( Akkermansia , Bifidobacterium , Roseburia , and Lactobacillus ) in CKD rats compared to inulin and chitosan oligosaccharide (Anegkamol et al., 2023)