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For experiments involving alterations to the culture medium, cells were seeded in regular RPMI 1640 with 5% dialysed FBS (Gibco, 26400044) and 1% penicillin and streptomycin

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This coordinated modulation can support capillary sprouting and vascular stabilization processes in experimental models

The cells were then washed five times for 2 min each with cold buffer

NOX1 expression correlates with RAS mutational status in colorectal cancer, and immunohistochemical analysis indicates overexpression in specific cancers, emphasizing its relevance as a therapeutic target in colorectal and small intestinal cancer

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