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Phospho(p)-IGF1R, MMP9, IL10, TNF and PD-1 levels were evaluated in homogenized lung tissue lysates using mouse ELISA kits, and normalized to total protein levels

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The tissue samples were taken during operations and placed directly into sterile Eppendorf plastic cryogenic vials or aspiration syringes, which were at once flash-frozen in a container of liquid nitrogen and transferred to a 80 C freezer

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In vivo studies also demonstrated the protective effects of liposome-encapsulated ferulic acid against oxidative liver damage (139)

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