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The amounts of albumin inside the lysed cells, or recycled albumin in the medium, were quantified by ELISA, in which albumin was captured on an anti-human albumin antibody and detected with an ALP-conjugated anti-human albumin antibody

During this process, glutathione transforms into glutathione disulfide, or oxidized glutathione (GSSG), and is regeneratedwith the help of an energy source like glucoseby the enzyme glutathione reductase (GR)

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It has since been used in clinical settings for fatigue, muscle spasm, upper respiratory conditions, and immune support

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