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At 0, 3, 7, and 10 days, the tissues were fixed, and cryosections were used for in situ apoptotic staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), according to the manufacturers instructions (Servicebio, Beijing, China)

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APAP hepatotoxicity results in early nitrotyrosine formation exclusively in hepatocyte mitochondria [41], suggesting that generation of elevated superoxide and its reaction with NO occurs within this organelle