To determine the optimal NaCl concentration to use as a diluent buffer for the triolein-substrate assay, we diluted PHP taken from four typical healthy subjects in KRP buffer containing 0.010.4 M NaCl and measured LPL activity using the triolein substrate assay described in Radiometric Detection of Lipoprotein Lipase Activity Using Triolein, ApoC-II, and Angiopoietin-Like 4. We found that LPL activity was highest when PHP was diluted in 0.175 M NaCl (Figure 3A), and therefore we recommend using 0.175 M NaCl when measuring LPL activity
Mitsumoto, H., Brooks, B
Hypoxia-Dependent HIF-1 Activation Impacts on Tissue Remodeling in Graves Ophthalmopathy-Implications for Smoking
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Less appetite often means fewer nutrients
Kesimer M, Ford AA, Ceppe A, Radicioni G, Cao R, Davis CW, Doerschuk CM, Alexis NE, Anderson WH, Henderson AG, et al
