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This potent compound serves as a remarkable activator of AMP-activated protein kinase (AMPK), a crucial enzyme responsible for regulating fat cell metabolism to burn fat for energy

Magnetic bead-based purification streamlines sample processing by eliminating column packing, centrifugation, and gravity-flow handling

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Then, the cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford a protein free lysate

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Because of this large size, negative charge, and polarity, NAD+ cannot readily cross cell membranes