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pJB-GST-HTS and the PCR fragment were prepared by BamHI and EcoRI digestion (Fw: AAAGGATCCATCATCATCATCATCATGGTCCGCTGGGCGTTCGTGGTATGGCTAGCAAAGGAGAAGAACTC, Rev: AAAGAATTCTCAGTTGTACAGTTCATCCATGCCATG)

This assertion is based on the clinical observation that patients with Hashimoto's thyroiditis often develop myxedema, and the knowledge that patients with myxedema due to atrophy of the thyroid have a high incidence of TG Ab in their serum

112 SheldonA

Critically, the peptide showed therapeutic efficacy even when administered after neurotoxin exposure

PMID: 36839273