The concentrated product was loaded onto a Superdex 200 increase 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5), 100 mM NaCl, 10 mM MgCl2, 100 M TCEP, 1 M GLP-1 or LY3502970 and/or 5 M compound 2, 0.00075% LMNG, 0.00025% GDN and 0.0002% (w/v) CHS
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Additional ingredients such as berberine and saffron extract have been studied for their roles in metabolic health and appetite perception
Circulation 124, 17741782
Retatrutide is being studied in phase 3 clinical trials following promising phase 2 results for weight reduction and metabolic improvement in adults with obesity, but it is not available through NHS prescribing pathways