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In each well of a poly-L-lysine coated 96-well plate (clear, GreinerBio, Kremsmnster, Austria), approximately 4,000 cells were seeded in 100 l Dulbeccos Modified Eagle Medium (DMEM) containing 10% fetal calf serum (FCS) and 1% penicillin and streptomycin (P/S)

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In an effort to come to the rescue of that breed, in 1973 a very knowledgeable and courageous breeder, Dr