Agnusdei D, Gentilella R
Bengtsson, G., Julkunen, A., Penttila, K
Mechanisms of action and therapeutic application of GLP-1. Cell Metabolism , 2021.
Zinman BA-O, Aroda VA-O, Buse JB, et al
Your BMI must exceed 30, or exceed 27 with at least one weight-related comorbidity such as type 2 diabetes, hypertension, dyslipidemia, or obstructive sleep apnea
The present invention relates in a still further aspect to a method for isolating a GLP-1 peptide, the method including purification of a GLP-1 peptide from a mixture comprising said peptide and related impurities via an anion exchange chromatography process, the anion exchange chromatography process comprising the step of: separating said GLP-1 peptide and said related impurities of said mixture by elution in a solution comprising an organic modifier, water, optionally a salt component, and optionally a buffer, with a linear or step gradient or isocratically in salt component and/or with a linear or step pH-gradient or at a constant pH-value, wherein the pH-gradient or pH-value should be in the range where said peptide has a negative local or overall net charge different from the local or overall negative net charge of said related impurities so as to remove said related impurities
