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P21 is an adamantane-containing synthetic peptide mimetic derived from Ciliary Neurotrophic Factor (CNTF) that operates via CNTF/JAK-STAT pathways

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Cells were fixed with 4% PFA for 30 minutes, permeabilized with 0.1% Triton X-100, and blocked with 5% normal goat or donkey serum

Importantly, these expanded HBs retained bipotency, co-expressing both hepatic and early cholangiocyte markers [1]

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