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The expression levels were normalized to the expression of the housekeeping gene RSP-9 (40S ribosomal protein S9) and then quantified using the 2 Ct method according to Livak and Smittgen 62 using StepOne v2.3 software (Applied Biosystems)

Esophageal variceal banding has also been used for that purpose, especially in patients who can't take beta blockers

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