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After the designated 6-h treatment time, the chorionic allantoic membrane (CAM) layer was carefully excised from the chick embryos, placed in ice-cold PBS, and homogenized using a Dounce homogenizer to ensure thorough disruption 59

It is rich in young stem cells, growth factors, and a natural protein scaffold

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For GLP-1 antagonist treatment, mice were injected with exendin-(939) amide (Ex-9) (2081, R&D Systems) at a dose of 25 nmol/kg/day or vehicle (PBS) for 5 days