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The reasons for Pure PRPs superior comfort profile include: Neutrophils in Regular PRP release inflammatory cytokines (TNF-, IL-1, IL-6) that prolong inflammation and increase pain[8][10] Red blood cells create viscosity in Regular PRP that makes injections more painful and traumatic[1][7] Hemoglobin release from damaged red blood cells causes tissue damage and oxidative stress[7] Pure PRPs cellular purity eliminates these pain-causing components entirely 2

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FAMEs were quantified using an Agilent Technologies 6890N gas chromatograph with a flame ionization detector (GC) [53]