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Notably, under BSO treatment, these molecules may increase cysteine availability but should not restore GSH levels, as BSO irreversibly inhibits -glutamylcysteine synthetase (-GCS) the enzyme responsible for GSH synthesis downstream of cysteine 48

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After 4 weeks of treatment, the levels of the microglia biomarker IBA1 and the extent of A plaque deposition were monitored using immunostaining

Streptavidin beads were washed twice with 1 ml of RIPA buffer and incubated with the cell lysate for 1 h at room temperature with rotation

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