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The tissue was then blotted dry and weighed followed by homogenization in 200 l buffer (0.05 M potassium phosphate and 0.1 mM EDTA, pH 7.8) and centrifuged at 15,000 xg for 30 min at 4C

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Interestingly, it was also found that carnitine increased the toxic effects of the thiol reducing agent DTT