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DAMPs also impact Kupffer cells, activating the nucleotide-binding oligomerization domain-like receptor (NLR) Family, Pyrin Domain-Containing 3 protein (NLRP3), a type of inflammasome leading to IL-1 secretion and inflammation, and pyroptosis, releasing more DAMPs and perpetuating a destructive cycle (Hammerich and Tacke, 2023)

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Studies using PPFCs usually target the analysis of cell morphology and alignment in ECs such as porcine aortic ECs [228] and HUVECs [229], and showed that the cells align gradually with the flow direction, morphologically change from a cobblestone aspect to an elongated shape, and alter intercellular space properties such as the increase in junction length

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