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Estradiol increases NO generation and endothelial function

The slides were then washed three times for 5 min

Nature, 268(5616), 152-153

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Obviously, you need to add a substrate - conventionally either of two substrates is used: malate, which is oxidised to oxaloacetate by reducing NAD succinate, which is oxidised to fumarate by reducing a flavin Perhaps less obviously, you also have to add ADP