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In a 2002 study by Anisimov et al., female transgenic FVB mice carrying the breast cancer gene HER-2/neu were given monthly injections of either vilon or epithalon

They also observed the reduced MG and oxidative stress under short term heat stress (42 C, 24 and 48 h)

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For the perfusions, mice were deeply anesthetized with isoflurane and perfused transcardially with saline, followed by a 10% buffered formalin solution (150200 mL per mouse)

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Shaking creates air bubbles and can denature the peptide