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The same non-specific peptide EF-GSA fused to the N-terminus of the receptor failed to strongly increase arrestin signaling compared with the non-specific peptide EF-GS, even when free EX4 was added (Figure 5d), providing further support that the C347K mutation changes GLP-1R to a G protein-biased receptor that favors G protein-associated signaling while it hinders the arrestin signaling

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