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Data acquisition and image processing The purified tirzepatideGIPRG s Nb35 complex at a concentration of 18-20 mg/mL was mixed with 100 M tirzepatide at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific)

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Pancreata were then incubated in 30% sucrose and tissue embedded medium (1:1) at 4 C overnight

When injected subcutaneously, this charge temporarily disrupts the pH balance of the surrounding tissue, triggering a brief localised inflammatory response

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