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ATCC CCL-185) cell lines were cultured at 37 C, 5% CO 2 in DMEM with 4.5 g/l of glucose, 1% penicillin/streptomycin (P/S) and 10% of heat-inactivated fetal bovine serum (FBS) (Gibco BRL, Gaithersburg, Md.)

The selection of an appropriate disruption method must be optimized for each biological source, as cell wall composition varies significantly between species, directly impacting the efficiency of this compound liberation

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