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Additionally, during the acridine orange (AO) reaction for a lysosome membrane permeability assay, the cells with indicated treatments were stained with 2 g/mL AO (Invitrogen) for 15 min at 37 C

59 In preclinical studies, it protects hepatocytes from lipotoxicity-induced apoptosis and prevents progression from simple steatosis to steatohepatitis

M., Ledesma-Pacheco, S