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[DOI] [PubMed] [Google Scholar] 73.Garcia R.J., Francis L., Dawood M., Lai Z.-W., Faraone S.V., Perl A

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Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

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Thus, current limitations concerning intralesional collagenase are of a high expenditure, and have an uncertain effect on specific patients with severe curvature and extensive calcification ( Topical Medication Topical approaches for drug delivery for PD were used due to its simplicity, painlessness, and safety

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