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TL;DR Reconstituting a lyophilised research peptide correctly means: pick the right diluent (bacteriostatic water with 0.9% benzyl alcohol), use the right volume based on your concentration maths, inject slowly down the inner vial wall, swirl gently (never shake), and store at 28C

doi: 10.2337/dci19-0014

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Previous studies have demonstrated that leptin dose-dependently inhibits glucose-stimulated insulin secretion in isolated pancreatic -cells, and significantly reduces insulin levels in perfused pancreatic preparations