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Growth of methionine synthesis mutants in laboratory cultures The L-methionine synthesis mutants were first grown in 7H9-Tween-ADC liquid medium, both with and without B12 (AdoCbl, 10 g/mL), to assess their growth in the presence and absence of the vitamin and thus validate their phenotypes

RNA interference To silence GLP-1R and PKA gene expression, cells were transfected with siRNAs using lipofectamine RNAi MAX reagent (Invitrogen)

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doi: 10.1016/j.gde.2014.06.005 102 NilssonEESkinnerMK

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