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Briefly, on Day 0, hiPSC colonies were dissociated with dispase and grown in a suspension to form embryoid bodies, and slowly transitioned to neural induction media (DMEM/F12 (1:1), 1% N2 supplement (Invitrogen), 1 x minimum essential media-nonessential amino acids (NEAAs), 2 g/ml heparin (Sigma))

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Account for this by adding 0.1-0.2 mL extra bacteriostatic water during reconstitution, or simply accept that the last draw from a vial may be slightly less than intended

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