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Intracellular lipid droplets were detected using a Lipid Droplet Green Fluorescent Assay Kit (Cat# C2053S, Beyotime Biotechnology) according to the instructions

Primary outcomes Behavioral analysis in areas of autism behavior and severity, communication, developmental status and behavioral problems were conducted at baseline visits 3 and 4 as well as at the end of the study during follow-up visits 6 and 7

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Supports cardiovascular health indirectly: Weight loss and glucose regulation together can reduce cardiometabolic risk factors