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2.3 Antibiotic persistence assay Pneumococcal strains were cultured in BHI until mid-log phase (OD 600nm ), exposed to 20 mM H 2 O 2 for 30 min, followed by treatment with 6 mg/mL levofloxacin for 5 hours at 37C

Timing: Take on an empty stomach between meals for best absorption

Longer term For longer or repeated protocols, review trends every 3 months with a clinician

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3.3.3 Bile acids Bile acids are synthesised in the hepatocyte by a complex multienzyme process (with at least 17 steps) from cholesterol