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Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)

Additionally, recognizing signs of degradation and understanding the potential health risks of expired medications are vital for safe usage

There was also a dose-response effect on the reduction in emptying rate

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