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Ice-cold MeOH (600 L), CHCl 3 (200 L), and H 2 O (500 L) samples were vortexed and then centrifuged (10,000 g , 10 min, 4 C) to precipitate proteins

Many communities have designated drop-off sites, household hazardous waste collection events, or mail-back programs for safe disposal of sharps containers

TUNEL assay Evaluation of apoptosis was performed using In Situ Cell Death Detection Kit, Fluorescein (Sigma-Aldrich) according to a previously published protocol (Abd-Elkareem et al., 2021)

IGF-1 LR3's extended half-life and reduced IGFBP binding create 23 higher free-fraction bioavailability compared to endogenous IGF-1, explaining its potent anabolic effect at lower microgram doses

6E) proteins was observed in endocrine-resistant breast tumors compared with primary ER+ breast tumors

Chi D, Singhal H, Li L, Xiao T, Liu W, Pun M