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For the OGD phase, cells were washed twice with PBS and then incubated in glucose-free DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) under hypoxic conditions (1% O 2 , 94% N 2 , 5% CO 2 ) in a hypoxia chamber (BioSpherix, Parish, NY, United States) for 8 h

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Enhancing robustness of spatial transcriptomic data through spatial imputation The limited number of features in the Xenium gene panel, while sufficient for cell segmentation, cell-type identification and mapping, constrains downstream analysis such cellcell communication (Supplementary Table 3 and Extended Data Fig

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