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The next generation of studies is, in that sense, as much a test of trial design and molecular delivery as of the hypothesis itself
IGF1, IGF2 and insulin act primarily through tyrosine-kinase-linked receptorsthe IGF1 receptor (IGF1R) and insulin receptor (IR)
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The proteins were purified from cell culture supernatant using a human albumin affinity column, followed by size exclusion chromatography (SEC), which yielded monomeric fractions that migrated on non-reducing sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels according to their molecular weight (MW) (66.5 kDa) (Fig
