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Seahorse assay Oxygen consumption rate was measured using a Seahorse Bioscience XF96 analyzer with an XF Cell Mito stress test kit (Agilent, 103015-100)

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Mechanistically, inhibition of complex I is expected to reduce mitochondrial ubiquinol (CoQ 10 H 2 ) levels, promoting lipid peroxidation and sensitizing cells to ferroptosis 107

Three groups were established with varying concentrations of astaxanthin (AX) in the diet and different temperature conditions: the NC group (0 mg/kg, 26 2C), the HTC group (0 mg/kg, 35 2C), and the HTV group (150 mg/kg, 35 2C)